Part of the TeachMe Series

The Zona Fasciculata

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Original Author(s): Will Clay
Last updated: 30th June 2020
Revisions: 11

Original Author(s): Will Clay
Last updated: 30th June 2020
Revisions: 11

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The Zona Fasciculata is the middle zone of the Adrenal Cortex, deep to the Zona Glomerulosa and superficial to the Zona Reticularis. It is the thickest of the three Zonas, measuring approximately 0.9mm and making up 50% of the mass of the Adrenal Gland.

In this article, we will discuss the cellular arrangement, function and clinical relevance of the Zona Fasciculata.

Structure

The Zona Fasciculata is made up of parenchymal cells known as spongiocytes, arranged into columns (sometimes called fascicles) with venous sinuses in between. Blood flows into the adrenal gland from the adrenal arteries, which are branches of the phrenic and renal arteries as well as the aorta. From here, blood flows through the adrenal tissue from superficial to deep, draining into sinusoids in the adrenal medulla and eventually into the central adrenal vein.

Fig 1 – Histology of the adrenal gland.

Function

The cells of the Zona Fasciculata secrete the glucocorticoids Cortisol and Corticosterone. These hormones regulate carbohydrate metabolism, particularly when an individual is in a time of stress (as part of the “fight-or-flight” response). In an adult human, approximately 10mg of Cortisol and 3mg of Corticosterone are secreted over a 24-hour period.

Fig 2 – Chemical structure of cortisol.

The synthesis pathway of the steroids secreted by the Zonas of the adrenal gland is complex. Cholesterol is the major precursor for all steroids secreted. The first step is initiated by the actions of ACTH and Angiotensin II activating adenylyl cyclase and phospholipase C respectively. Cholesterol can then be converted to a steroid called Pregnenolone via an enzyme of the cytochrome P450 superfamily called cholesterol desmolase.

From Pregnenolone, all the major secreted mineralocorticoids, glucocorticoids and androgens can be synthesised in a multi-step enzyme-assisted pathway. The metabolites of the synthesis pathway are moved in and out of the mitochondria, the smooth endoplasmic reticulum and the cytoplasm.

It is the presence or lack of specific enzymes in each Zona that determines which hormones are secreted. In the Zona Fasciculata, the enzyme 11β-hydroxylase catalyses the final step of the reaction that forms Cortisol and Corticosterone.

Additionally the secretion of cortisol follows a diurnal pattern with more being secreted in the mornings.

Clinical Relevance – Cushing’s Syndrome

In a steroid-producing adrenal tumour (or Anterior Pituitary adenoma), large concentrations of glucocorticoids are secreted in the body. When in high concentrations, glucocorticoid steroids activate mineralacorticoid receptors due to the similarity in shape of the receptors.

Therefore, a patient with Cushing’s Syndrome will have symptoms of high glucocorticoid secretion (fat build-up on back of neck and around face, wasting of limb muscles with central obesity, purple striae, hyperpigmentation) as well as show effects of high mineralocorticoid concentrations (hypertension, hypokalaemia).

Treatment of Cushing’s depends on the underlying cause, for example a pituitary adenoma may be surgically removed, as can a metastatic lung tumour secreting ACTH.

Fig 4 – Diagram showing the symptoms of Cushing’s syndrome.

Clinical Relevance – Addison’s disease

Addison’s disease is the opposite disease to Cushing’s disease in many ways. Whereas Cushing’s disease is due to an excess of cortisol, Addison’s disease is due to a lack of cortisol, commonly due to autoimmune destruction of the adrenal cortex. This in turn causes hypotension and anorexia, in contrast to hypertension and truncal obesity in Cushing’s disease.

A unique symptom of Addison’s disease is hyperpigmentation, particularly in the creases of the hand and in the mouth. This can be explained via the hypothalamic – pituitary – adrenal (HPA) axis. The decrease in cortisol leads to an increase in ACTH via negative feedback.

ACTH stems from a precursor molecule called pro-opiomelanocortin (POMC). POMC is also a precursor to Melanocyte Stimulating Hormone (MSH), causes darkening of skin. Therefore, an increase in ACTH, leads to an increase in POMC and as a byproduct, an increase in MSH and therefore leads to skin darkening.

A very serious complication of Addison’s disease is an Addisonian crisis. As discussed above, cortisol is linked to the “fight or flight” response and is released in times of stress to the body.

Therefore, in patients suffering from Addison’s disease, they are unable to mount an adequate response to these stresses. It is a life threatening complication, resulting in numerous symptoms such as severe hypotension and electrolyte dysfunction.

Clinical Relevance – Enzyme Deficiencies

A deficiency in the enzyme 3β-hydroxysteroid dehydrogenase causes an increased secretion of DHEA – a weak androgen that causes masculinisation in females but is not strong enough to drive male genital development alone. As such, male neonates with this deficiency are likely to have hypospadias, where the opening of the urethra is found on the underside of the penis rather than at the tip.

The most common adrenal enzyme deficiency is 21β-hydroxylase deficiency, making up 90% of the deficiency cases. Production of Cortisol and Aldosterone are reduced, causing a raised ACTH secretion. Precursor steroids are converted to androgens which then drive masculinisation, although this may not become apparent until later life. The lack of aldosterone results in massive loss of Na+ which manifests as severe hypovolaemia.