Prolactin

Written by Neil Thakrar, Kishan Pankhania and Elly Voogt

Last updated 18th May 2026
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Prolactin is one of the hormones produced by the anterior pituitary gland. It is particularly important in breast development and production of breast milk during pregnancy and breastfeeding.

In this article, we will look at the production and regulation of prolactin, along with its physiological functions and related clinical conditions.

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Prolactin Production

Prolactin (PRL) is produced by lactotroph cells in the anterior pituitary gland. Unlike many other anterior pituitary hormones, prolactin secretion is primarily controlled by inhibition rather than stimulation from the hypothalamus.

Functions of Prolactin

The main physiological functions of prolactin are related to pregnancy and lactation. During pregnancy, gradually increasing prolactin levels act alongside oestrogen and progesterone to promote breast development.

The main actions of prolactin include:

  • Initiation of milk production by alveolar cells (lactogenic effect)
  • Maintenance of milk production after lactation has started (galactopoietic effect)
  • Proliferation and growth of alveolar and duct cells (mammogenic effect)

Prolactin’s most important physiological roles are the lactogenic and galactopoietic effects involved in breastfeeding. Oxytocin is also involved in breastfeeding by stimulating milk ejection from the breast, whereas prolactin primarily stimulates milk production.

Fig 1
Diagram showing the structure of a lactating breast

Regulation of Prolactin Secretion

Prolactin release is inhibited by dopamine (DA), and is therefore known, in this context, as prolactin inhibitory factor (PIF).

Suckling is the strongest physiological stimulus for prolactin release. Stimulation of the nipple sends signals via afferent neural pathways through the spinal cord. These signals inhibit dopaminergic neurons in the hypothalamus. Since, dopamine normally suppresses prolactin secretion, this process increases prolactin release through a processes called disinhibition.

Thyrotropin-releasing hormone (TRH), produced by the hypothalamus, also stimulates prolactin secretion. Furthermore, oestrogen increases the sensitivity of lactotroph cells to TRH, while also reducing their sensitivity to dopamine inhibition.

Diagram showing prolactin regulation with stimulation of the anterior pituitary by TRH released by the hypothalamus to increase secretion and inhibition of the anterior pituitary by hypothalamic dopamine. Prolactin is shown to act on the target breast tissue, whilst suckling inhibits the dopaminergic neurones, increasing prolactin release

Fig 2
Diagram showing the regulation of prolactin secretion

Note: the prolactin axis does not technically have a negative feedback system. Without inhibition by dopamine, prolactin would be secreted indefinitely.

Clinical Relevance

Breastfeeding and Ovarian Cycles

Lactation commonly suppresses normal ovarian cycles after pregnancy. This occurs because prolactin reduces gonadotropin-releasing hormone (GnRH) secretion from the hypothalamus. Reduced GnRH secretion decreases follicle-stimulating hormone (FSH) and luteinising hormone (LH) release, therefore suppressing ovulation.

Breastfeeding will not indefinitely cease ovarian cycles. Cycles eventually resume even if breastfeeding continues. If the mother does not breastfeed the infant, ovulatory cycles will usually resume within 8-10 weeks after delivery.

Clinical Relevance

Hyperprolactinaemia

Hyperprolactinaemia refers to abnormally elevated prolactin levels.

Common clinical features include:

  • Menstrual disturbances such as amenorrhoea or oligomenorrhoea
  • Galactorrhoea (milk discharge unrelated to breastfeeding)
  • Hypogonadism, which can cause infertility, erectile dysfunction and osteoporosis

The causes of hyperprolactinaemia can be remembered using the 5 Ps:

Physiological Breastfeeding, stress, transient rises after intercourse
Pregnancy Due to increased oestrogen stimulation of prolactin secretion
Pharmacological Particularly dopamine antagonists, such as antipsychotic medications
Prolactinoma A prolactin-secreting pituitary tumour
Polycystic Ovarian Syndrome (PCOS) The mechanism is not exactly known but is thought to be related to increased oestrogen stimulation of prolactin secretion

Management involves identifying and treating the underlying cause.

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